Khảo sát khả năng ứng dụng kỹ thuật nuôi cấy mô sẹo, tạo dịch huyền phù cây Drosera burmanni Vahl trong thu nhận hợp chất anthraquinone

v
SUMMARY

The thesis         
Drosera burmanni  was carried out
at Plant Biotechnology lab, University of Natural Sciences; and Chromatography
room, Research Institute for Biotechnology and Enviromental Technology,
University of Agriculture and Forestry, HCM city from March to August, 2007.

Results:
- Callus of D. burmanni Vahl was cultured successfully by using
Gamb 
2,4-D (0,2 mg/l), succrose (20g/l), PVP (1,25 mg/l), pH was adjusted to
5,8 before autoclaving.
- Column Chromatography and Thin Layer Chromatography were used to
purify anthraquinone compound in D. burmanni. This compound was
used as authentic sample for HPLC technique.
- Roots of D. burmanni contained 3,01% dry weight (DW) of anthraquinone,
larger than 2,78% in leaves.
- Anthraquinone content of red-leaf D. burmanni Vahl took 0,78% of DW,
compared with 2,78% DW in green-leaf trees.
- Biomass of D. burmannis suspension contained 0,02% of fresh weight of
anthraquinone, while no quantifiable amounts of anthraquinone has been
found in spent medium.

Ho Chi Minh city, September, 2007.
Hoang Thi Thu


vi





 .i




 iii
 iv
Summary v


 vi






 ix






 x




 xii






 xiii

 1
1.1.  1
1.2.  2
1.3.  2
1.4.  2
 3
2.1. Drosera burmanni Vahl 3
2.1.1.  3
2.1.2.  3
2.1.3.  4
2.1.4.  6
2.1.5.  7
2.1.6.  8
2.1.7.  11
2.1.8.  12
2.1.8.1.  12
2.1.8.2.  12
2.1.8.3.  12
2.1.8.4.  14
2.1.8.5.  15
2.1.9.  16

h 17
2.2.1.  17
2.2.2.  18
2.2.2.1.   18
2.2.2.2.  19
2.2.3.  19
2.2.3.1.  19
2.2.3.2.  19


vii
2.2.3.3.  20
2.2.3.4.  20
2.2.3.5. 
 22
2.2.4. 
 22
2.2.4.1.  22
2.2.4.2.  22
2.2.4.3.  22
2.2.4.4. o 23
2.2.4.5.  23
2.2.4.6.  23
2.3.  24
2.3.1.   24
2.3.1.1.  24
2.3.1.2.  26
2.3.1.3.  27
2.3.2.  28
  31
 31
3.1.  31
3.1.1.  31
3.1.2.  33
3.1.2.1. 
 33
3.1.2.2. 
 34
3.1.2.3. 3: 

2,4-
 35
3.1.2.4. 
 36
3.1.2.5. 
 37
3.2. 







 D. burmanni 38
3.2.1.  38
3.2.2.  39

in vitro  40
 40
 40
 40
 41


viii
ong
 41

in vitro 43

 44

 45
 45
-  46
4.1.  46

 46

 48
4.1.3. 3: 

2,4-
 49

 54
4.1.5. 5: 
 56
4.2. 







 D. burmanni 58
4.3. 
 59
 59



 61

in vitro 62

 64

 66
  68
5.1.  68
5.1.1.   68
5.1.2.  68
5.2.  69

 70



ix












2,4-D : 2,4-dichlorophenoxy acetic acid
BAP : 6-benzylaminopurine
CRD : Completely Randomized Design ()

HPLC : High Performane Liquid Chromatography (





)
IAA : 3-indolyl-acetic acid
IBA : 3-indolebutyric acid
MS : Murashige & Skoog, 1962
NAA : naphthalene acetic acid
PVP : poly vinyl pyrrolidone
TCL : Thin Cell Layer (





)
TLC : Thin Layer Chromatography (





)


x




TRANG
Drosera burmanni  4
nh  Drosera burmanni Vahl 4
D. burmanni 5
nh 2.4. D. burmanni Vahl  7
 10
2.6. 



Drosera  15
 21
 21
2.9. 



 24
2.10. 





 26
 27
2.12. 













 29
D. burmanni in vitro 31
4.1. 















D.burmanni 


5 

(0,2 mg/l) 2,4-






i 52
4.2. 


















D. burmanni 

5 

 (0,2 mg/l) 2,4-D






 53
 55
  55
 55
4.4. 


 D. burmanni 57
pm 60
 60


xi
 60
 60


70 ppm 60
D. burmanni 
 61
D. burmanni 
 61
D. burmanni Vahl 63
4.13. 

D. burmanni Vahl 63
D. burmanni  65
D. burmanni 
 65
 
 66
4.17.  66


xii












TRANG



2.1. 

















 18
3.1. Quy t 39
3.2. 







 42
3.3. 









45
3.4. 









(

) 58








4.1
 46


 54



 59


xiii




TRANG
 Drosera 
7-methyljuglone 8
 Drosera 9

Drosera .13

v 

 33

 34
ng 3.3. 

2,4-D
 35

 36

 37

in vitro 43

c  44
  46

 47
 
 48
 
 48


xiv

 49

 54
 56
 59
 
in vitro 62
4.10. 










64
4.11. 







66

Xem chi tiết: Khảo sát khả năng ứng dụng kỹ thuật nuôi cấy mô sẹo, tạo dịch huyền phù cây Drosera burmanni Vahl trong thu nhận hợp chất anthraquinone